rabbit anti mouse myo d polyclonal antibody  (Bioss)

Journal: The Journal of Biological Chemistry

Article Title: A-to-I RNA editing impairs miR-376b-3p repression of RYBP in skeletal muscle satellite cells

doi: 10.1016/j.jbc.2025.111006

Figure Lengend Snippet: Effects of WT and edited miR-376b-3p on the differentiation of goat MuSCs . A , expression of miR-WT and miR-E following transfection with miR-A, miR-G, or miR-NC in MuSCs, and the data were evaluated using the one-way ANOVA. Data are represented as the mean ± SD, n = 3 independent experiments. B , mRNA expression of differentiation markers MyoD (an early myogenic determination factor), MyoG (a marker of myoblast differentiation), and MyHC (indicative of terminal differentiation and myotube formation) following transfection with miR-A, miR-G, or miR-NC in MuSCs. Data were analyzed using one-way ANOVA and are presented as the mean ± SD (n = 3 independent experiments). C and D , Western blot analysis of MyoG, MyoD, and MyHC protein levels in MuSCs transfected with miR-A, miR-G, or miR-NC, and the data were evaluated using the one-way ANOVA. Data are represented as the mean ± SD, n = 2 independent experiments. E , immunofluorescence staining of MyHC to evaluate the differentiation potential of MuSCs after transfection with miR-A, miR-G, or miR-NC. The fusion index is quantified on the right , and the data were evaluated using the one-way ANOVA. Data are represented as the mean ± SD, n = 6 independent experiments. Asterisks indicate statistical significance: ∗ for p < 0.05, ∗∗ for p < 0.01, and “ns” for nonsignificant differences. MuSC, skeletal muscle satellite cell.

Article Snippet: Membranes were blocked and incubated overnight at 4 °C with the appropriate primary antibodies Pax7 (1:200 dilution; Santa Cruz), PCNA (1:1000 dilution; Abclonal), MyoG (1:1000 dilution; Bioss), MyoD (1:1000 dilution; Proteintech), MyHC (1:1000 dilution; Zen Bio), and β-tublin (1:2000 dilution; Zen Bio), followed by incubation with horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution; Zen Bio) at 37 °C for 2 h. Protein signals were visualized using enhanced chemiluminescence reagents (Pierce). β-tubulin was used as a loading control.

Techniques: Expressing, Transfection, Marker, Western Blot, Immunofluorescence, Staining

Journal: The Journal of Biological Chemistry

Article Title: A-to-I RNA editing impairs miR-376b-3p repression of RYBP in skeletal muscle satellite cells

doi: 10.1016/j.jbc.2025.111006

Figure Lengend Snippet: Effects of miR-376b-3p and its edited form and RYBP overexpression on MuSC differentiation . A , quantification of cotransfection efficiency of RYBP , miR-WT, and miR-E at the differentiation stage of MuSCs, and the data were evaluated using the one-way ANOVA. Data are represented as the mean ± SD, n = 3 independent experiments. B , mRNA expression of myogenic differentiation markers following cotransfection of pRYBP with miR-A or miR-G, and the data were evaluated using the one-way ANOVA. Data are represented as the mean ± SD, n = 3 independent experiments. C and D , Western blot analysis of MyoG, MyoD, and MyHC protein levels in MuSCs after cotransfection with pRYBP and miR-A or miR-G, and the data were evaluated using the one-way ANOVA. Data are represented as the mean ± SD, n = 2 independent experiments. E , immunofluorescence staining of MyHC to assess myotube formation after cotransfection with pRYBP and miR-A or miR-G. The fusion index is shown on the right , and the data were evaluated using the one-way ANOVA. Data are represented as the mean ± SD, n = 6 independent experiments. Asterisks indicate statistical significance: ∗ for p < 0.05, ∗∗ for p < 0.01, and “ns” for nonsignificant differences. MuSC, skeletal muscle satellite cell; pRYBP, RYBP plasmid; RYBP, Ring1 and YY1 binding protein.

Article Snippet: Membranes were blocked and incubated overnight at 4 °C with the appropriate primary antibodies Pax7 (1:200 dilution; Santa Cruz), PCNA (1:1000 dilution; Abclonal), MyoG (1:1000 dilution; Bioss), MyoD (1:1000 dilution; Proteintech), MyHC (1:1000 dilution; Zen Bio), and β-tublin (1:2000 dilution; Zen Bio), followed by incubation with horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution; Zen Bio) at 37 °C for 2 h. Protein signals were visualized using enhanced chemiluminescence reagents (Pierce). β-tubulin was used as a loading control.

Techniques: Over Expression, Cotransfection, Expressing, Cell Characterization, Western Blot, Immunofluorescence, Staining, Plasmid Preparation, Binding Assay